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A metabolic pathway that can be either catabolic or anabolic depending on energy availability is called an amphibolic pathway,; this path is thought to facilitate muscle growth and maintenance in obese animal models . A high relative energy intake is associated with faster weight gain and longer period weight gain in adult mice ,. Moreover, animals subjected to an elevated fat diet show a rapid decrease in fat-free mass , suggesting that a rapid fat-free mass loss may contribute to obesity and the development of overweight and obesity-type behaviors. However, despite this, there is little knowledge about how these behavioral changes may be affected by the composition of the diet. Therefore, we used the data from 10,000 adult mice to assess the effects of altering the fatty acid composition, but not the fat content, of the diet on behaviors and gene expression. Plasma FFAs are associated with insulin-like growth factor-I (IGF-I)-induced growth of adipocytes as well as growth of adult human adipocytes as evidenced by insulin-like growth factor receptors (IGRs) (data not shown). As adipocytes and adipocytes express IGRs, they may be critical components of the energy homeostasis that may also have an effect on the induction of lean tissue, and adipocyte size. We did not observe significant alterations in adipogenic hormones in control diets during diet composition. This is because leptin, which is suppressed in obese individuals, was not observed to alter IGR expression or growth in mice fed a high fat diet and/or a high carbohydrate diet. Furthermore, we did not find changes in leptin expression, IGF-I, and growth hormone (GH) levels that would indicate any change in the metabolic state. In this study, mice fed diets higher in carbohydrate than fat were also fed a high fat-acid diet, which suggested that insulin-like growth factor-I may influence fat metabolism, growth regulation, and fat-free mass. Although the high fat-acid diet appears to promote adipose tissue development and insulin resistance, the mechanism in vivo and in animal models is uncertain. It is also unclear from our data if obesity affects the expression of different types of insulin-like growth factor-I receptors as well as type I and II adipose tissue. We did not find changes in adipogenic hormone levels in mice fed a low fat-acid diet. In addition, we did not detect changes in IGF-I, IGRs, GH, or AAT concentrations in mice fed a high fat-acid diet or diets high in fat-isocaloric carbohydrate Related Article: